| 作者： | Wei Wang, Li Qin, Wenjing Zhang, Linghui Tang, Chao Zhang, Xiaojing Dong, Pei Miao, Meng Shen, Huilong Du, Hangyuan Cheng, Ke Wang, Xiangyun Zhang, Min Su, Hongwei Lu, Chang Li, Qiang Gao, Xiaojuan Zhang, Yun Huang, Chengzhi Liang, Jian-Min Zhou, and Yu-hang Chen |
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| 刊物名称： | Cell |
| DOI： | 10.1016/j.cell.2023.05.023 |
| 摘要： | Plant roots encounter numerous pathogenic microbes that often cause devastating diseases. One such pathogen, Plasmodiophora brassicae (Pb), causes clubroot disease and severe yield losses on cruciferous crops worldwide. Here, we report the isolation and characterization of WeiTsing (WTS), a broad-spectrum clubroot resistance gene from Arabidopsis. WTS is transcriptionally activated in the pericycle upon Pb infection to prevent pathogen colonization in the stele. Brassicanapus carrying the WTS transgene displayed strong resistance to Pb. WTS encodes a small protein localized in the endoplasmic reticulum (ER), and its expression in plants induces immune responses. The cryo-EM structure of WTS revealed a previously unknown pentameric architecture with a central pore. Electrophysiology analyses demonstrated that WTS is a calcium-permeable cation-selective channel. Structure-guided mutagenesis indicated that channel activity is strictly required for triggering defenses. The findings uncover a unique ion channel analogous to resistosomes that triggers immune signaling in the pericycle. |
